mouse anti-human cd55 (Millipore)
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mouse anti-human cd55
Mouse Anti Human Cd55, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-human+cd55/mouse+anti+human+cd55/us12049512-1698-30-37
Average 90 stars, based on 1 article reviews
Mouse Anti Human Cd55, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-human+cd55/mouse+anti+human+cd55/us12049512-1698-30-37
Average 90 stars, based on 1 article reviews
mouse anti-human cd55 - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Antibody variants and uses thereof Article Snippet: To compare the expression levels of complement regulatory proteins between these cell lines, QIFIKIT analysis was performed to determine the levels of Article Title: Antibody variants and uses thereof Article Snippet: To compare the expression levels of complement regulatory proteins between these cell lines, QIFIKIT analysis was performed to determine the levels of Article Title: Incorporation of Host Complement Regulatory Proteins into Newcastle Disease Virus Enhances Complement Evasion Article Snippet: After blocking, adsorbed viruses were probed with |
Figure S1 and 
Table S1 . The score for each protein is defined as the overall enrichment for that corresponding gene (relative to the unselected library) multiplied by the percentage of barcodes associated with the enriched gene (defined as a log fold change [logFC] of >0). The dotted line reflects a BASEHIT score of 1, and all genes that had higher scores are listed in Table S1. (C) CD55 interactions with different Borrelia species. Samples from 29 Borrelia isolates were screened against the host BASEHIT protein library. The y axis represents the CD55 score as the overall enrichment for CD55 of each isolate (relative to the unselected library) multiplied by the percentage of barcodes associated with the enriched CD55 (defined as logFC of >0). " width="250" height="auto" />
Figure S2 , 
![CD55 binding to B. crocidurae and its impact on complement inhibition. (A and B) Binding of human and mouse CD55 to B. crocidurae . (A) B. crocidurae cultures were grown to a density of 10 5 CFU/mL and incubated with recombinant human CD55-Fc (50 μg/mL) alone or in the presence of mouse CD55-His 8 (100 μg/mL). B. crocidurae ’s binding to human CD55-Fc was measured by flow cytometry using a human CD55 monoclonal antibody (MAB2009; R&D systems) and goat anti-mouse Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific) (1:1,000). The results from one of five representative experiments are shown here. (B) Data from five independent experiments are shown here. The y axis represents arbitrary fluorescence units (mean fluorescence intensity of AF488) from the B. crocidurae spirochete population. Data were acquired on a BD LSRII flow cytometer and analyzed by FlowJo software. The bars represent mean values ± standard deviations (SD), and P values were determined by the Student t test (Mann-Whitney test of the data for B. crocidurae incubated with hCD55 Fc versus mCD55/hCD55Fc [ P = 0.0079]). (C and D) Binding of mouse CD55 to proteinase K-treated or untreated B. crocidurae spirochetes. (C) B. crocidurae cultures were grown to a density of 10 5 CFU/mL and incubated in the presence or absence of proteinase K (0.2 mg/mL) at 37°C for 10 min. Subsequently, the proteinase K activity was quenched using a Roche cOmplete proteinase inhibitor cocktail and spirochetes were washed with PBS thrice. Borrelia spirochetes were incubated with recombinant mouse CD55-His 8 (50 μg/mL). B. crocidurae ’s binding to mouse CD55 was measured by flow cytometry using a secondary AF488-conjugated anti-His 6 monoclonal antibody. Background binding of AF488-conjugated anti-His 6 monoclonal antibody alone with B. crocidurae is shown by the gray-shaded region. The results from one of five representative experiments are shown here. (D) Data from five independent experiments are plotted here. The y axis represents the mean fluorescence intensities of AF488 from B. crocidurae . Statistical significance was assessed using the nonparametric Student t test (Mann-Whitney test of the data for proteinase K-pretreated B. crocidurae incubated with mCD55 versus untreated B. crocidurae incubated with mCD55 [ P = 0.0079]). (E) Complement-mediated killing of B. crocidurae in the presence or absence of recombinant human CD55. Human CD55 (100 μg/mL) was incubated with B. crocidurae for 2 h in the presence or absence of immune serum from mice that were infected 30 days previously with B. crocidurae . Viability was assessed by observing spirochete movement under dark-field microscopy. The growth inhibition of B. crocidurae was calculated based on the growth of untreated B. crocidurae . The bars represent mean values ± SD, and P values were determined by the Student t test (Mann-Whitney test of the data for 20% serum versus 20% serum plus hCD55 [ P = 0.0023] or 40% serum versus 40% serum plus hCD55 [ P = 0.0023]). Data from seven independent experiments are shown here. (F) Complement-mediated killing of B. crocidurae in the presence or absence of recombinant mouse or human CD55. Human or mouse CD55-His 8 (100 μg/mL) was incubated with B. crocidurae for 24 h in the presence or absence of 20% serum from mice that were infected 30 days previously with B. crocidurae . Viability was assessed using the Bac-Titer Glo assay. The growth inhibition of B. crocidurae was calculated based on the growth of B. crocidurae incubated with PBS alone. Statistical significance was assessed using the Student t test (PBS versus mCD55 [ P = 0.0132] or PBS versus hCD55 [ P = 0.0105]). Data from four independent experiments performed in triplicates are shown here.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0505/pmc09600505/pmc09600505__mbio.01161-22-f003.jpg)
Fig. S4 ). (B) B. crocidurae interactions with erythrocytes from WT and CD55 KO mice were compared. Erythrocytes from WT and CD55 KO mice were incubated with 10 6 B. crocidurae spirochetes in 0.2-mL PCR tubes at 37°C as described before . Hemoglobin from erythrocytes that interacted with B. crocidurae was quantified using the QuantiChrom hemoglobin assay kit. Data from three independent experiments performed in triplicates are presented. (C) B. crocidurae ’s interaction with human RBCs in the presence of anti-CD55 neutralizing antibody (R&D Systems) was measured. Human RBCs were preincubated with either anti-human CD55 neutralizing antibody or another antibody (anti-CD3 antibody) as an isotype control for 20 min. The RBCs were incubated with 10 6 B. crocidurae spirochetes. The interactions between human RBCs and B. crocidurae spirochetes were measured by flow cytometry. Data from three independent experiments are presented. (D) B. crocidurae ’s interactions with RBCs in the presence of recombinant CD55 or PGLYRP1 were compared. RBCs from C57BL/6 mice were incubated with 10 6 B. crocidurae spirochetes at 37°C in the presence or absence of recombinant human (h) or mouse (m) CD55 (100 μg/mL). Hemoglobin from RBCs that interacted with B. crocidurae was quantified. " width="100%" height="100%">
